Health Articles - Anti-Müllerian Hormone (AMH) Induces Aromatase Expression in Human Granulosa Cells - A study

by ANIRBAN BHATTACHARYA - Date: 2009-07-14 - Word Count: 338 Share This!

Objective: Anti-Müllerian Hormone (AMH) is a member of the transforming growth factor-? (TGF-?) family. Within the ovary AMH is produced solely by granulosa cells and plays an important role in regulation of ovarian follicle growth. In mouse ovary, AMH has been found to inhibit recruitment of primordial follicles into the pool of growing follicles and decreases the sensitivity of small pre-antral follicles to FSH. Along with its pivotal role in follicular development, AMH serves as a putative marker of ovarian reserve and of assisted reproductive technology outcome. Aromatase cytochrome P450 (encoded by the CYP19 gene) is the key enzyme in the biosynthesis of estrogens from C19-steroid precursors. Previous animal studies have shown that AMH inhibits the expression of aromatase in fetal ovaries; however, the effect of AMH on aromatase in postnatal human ovaries is not well understood. The objective of the present study was to investigate the effects of AMH on the expression of aromatase in human granulosa cells (hGCs).

Design: Effects of AMH in the absence and presence of cAMP were studied in primary luteinized hGCs obtained during In vitro fertilization cycles and in the immortalized human granulosa cell line, HGL5.

Materials and Methods: hGCs were cultured +/- dibutyryl cAMP (1 mM), +/- AMH (5-100 ng/ml) for 24-48h. RNA was isolated, reversed-transcribed and real-time PCR was performed to measure mRNA transcripts for aromatase containing the ovary-specific first exon (CYP19IIa) and for AMH.

Results: In HGL5 cells, cAMP caused a ~40-fold induction of CYP19IIa mRNA. While AMH (25 ng/ml) alone had a modest stimulatory effect (~1.5-fold), co-treatment with AMH + cAMP caused a synergistic ~75-fold increase in CYP19IIa mRNA levels. In primary cultures of hGCs, basal CYP19IIa mRNA levels were elevated; cAMP caused a ~2.5-fold increase in CYP19IIa mRNA, while AMH had a moderate stimulatory effect (1.5-fold). In cells co-treated with AMH + cAMP, CYP19IIa mRNA levels were increased ~3.5-fold.

Myself webmaster of , a service provider of assured fertility solutions, specializes in Tubal Reversal Treatment, tubal reversal, Fertility Clinics, Egg Donation, ivf cost, Fertility Doctor, Gestational Surrogacy, and embryo transfer

Related Tags: fertility clinics, in vitro fertilization, tubal reversal, ivf cost, in vitro diagnostics, tubal reversal treatment, egg donation, fertility doctor

Your Article Search Directory : Find in Articles

© The article above is copyrighted by it's author. You're allowed to distribute this work according to the Creative Commons Attribution-NoDerivs license.

Recent articles in this category:

Most viewed articles in this category: