Health Articles - Anti-Müllerian Hormone (AMH) Induces Aromatase Expression in Human Granulosa Cells - A study
Design: Effects of AMH in the absence and presence of cAMP were studied in primary luteinized hGCs obtained during In vitro fertilization cycles and in the immortalized human granulosa cell line, HGL5.
Materials and Methods: hGCs were cultured +/- dibutyryl cAMP (1 mM), +/- AMH (5-100 ng/ml) for 24-48h. RNA was isolated, reversed-transcribed and real-time PCR was performed to measure mRNA transcripts for aromatase containing the ovary-specific first exon (CYP19IIa) and for AMH.
Results: In HGL5 cells, cAMP caused a ~40-fold induction of CYP19IIa mRNA. While AMH (25 ng/ml) alone had a modest stimulatory effect (~1.5-fold), co-treatment with AMH + cAMP caused a synergistic ~75-fold increase in CYP19IIa mRNA levels. In primary cultures of hGCs, basal CYP19IIa mRNA levels were elevated; cAMP caused a ~2.5-fold increase in CYP19IIa mRNA, while AMH had a moderate stimulatory effect (1.5-fold). In cells co-treated with AMH + cAMP, CYP19IIa mRNA levels were increased ~3.5-fold.
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